MKTTn Broth is used as one of two selective enrichment medium, along with RVS broth, for salmonellae in milk and dairy products and in other food products following the horizontal method described in the ISO 6579 standard. Associated with MSRV medium, it is also used in the protocol for the isolation and identification of Salmonella in animal production environments, in poultry and in mammals.
MKTTn broth is also used as a second selective enrichment broth for the detection of Salmonella in waters.
TYPICAL COMPOSITION
The composition can be adjusted in order to obtain optimal performance.
For 1025 mL of media :
- Tryptone ...................................................................................................................... 8,6 g
- Meat extract................................................................................................................. 4,3 g
- Bile salts .................................................................................................................... 4,78 g
- Sodium chloride .......................................................................................................... 2,6 g
- Calcium carbonate .................................................................................................... 38,7 g
- Sodium thiosulfate, anhydrous* .............................................................................. 30,45 g
- Brillant green ............................................................................................................ 9,6 mg
- Iodine........................................................................................................................... 4,0 g
- Potassium Iodide......................................................................................................... 5,0 g
- Novobiocin................................................................................................................. 40 mg
For 89,5 g of dehydrated base BK208
- Tryptone ...........................................................8,6 g
- Meat extract .........................................................4,3 g
- Bile salts .........................................................4,78 g
- Sodium chloride ...................................................2,6 g
- Calcium carbonate .............................................38,7 g
- Sodium thiosulfate, anhydrous*.......................30,45 g
- Brilliant green ....................................................9,6 mg
- Novobiocin .........................................................40 mg
* NOTE : 30.45g of Sodium thiosulfate, anhydrous is equal to 47.8 g Sodium thiosulfate pentahydrate
PACKAGING
Ready-to-use medium:
BM07808 - 50 tubes of 10 mL
Dehydrated medium (without iodine):
BK208HA - 500 g bottle
Dehydrated medium (without iodine nor Novobiocin):
BK169HA - 500 g bottle
Novobiocin Selective Supplement :
BS03308 - 10 vials of 10 mg
BS05608 - 8 vials of 40 mg
SELECTIVE ENRICHMENT OF SALMONELLA