DESCRIPTION 

Thioglycollate Medium with Resazurin is used for sterility tests of biological products and for the culture of aerobic, anaerobic and microaerophilic bacteria. The formulation of the medium complies with the requirements of the European Pharmacopoeia, the United States Pharmacopoeia and the AOAC for the bacteriological analysis of antibiotics and the determination of the sporicidal effect of disinfectants. This medium is also used for the confirmation of Clostridium perfringens in food products.  

TYPICAL COMPOSITION   

The composition can be adjusted in order to obtain optimal performance.   

For 1 liter of media :

- Tryptone .................................................................................................................. 15,00 g

- Yeast extract ............................................................................................................. 5,00 g

- Glucose monohydrate ............................................................................................... 5,50 g

- Sodium chloride ........................................................................................................ 2,50 g

- Sodium thioglycollate ................................................................................................ 0,50 g

- L-cystine .................................................................................................................... 0,50 g

- Resazurin ................................................................................................................. 1,0 mg

- Bacteriological agar ................................................................................................... 0,75 g 

pH or the ready-to-use media at 25 °C : 7,1 ± 0,2. 

PACKAGING   

Dehydrated media : 

500 g bottle .......................................................................................................... BK017HA 

Ready-to-use media :

50 x 10 mL tubes ......................................................................................................... BM08208