DESRIPTION
Bismuth Sulfite (modified Wilson Blair agar) Agar is a selective medium used to isolate Salmonella Typhi and other salmonellas in pathological products of animal origin, water, dairy and other food products. Bismuth Sulfite agar can be used in the normalized methods for Salmonella detection as the second isolation media.
TYPICAL COMPOSITION
The composition can be adjusted in order to obtain optimal performance.
For 1 liter of media :
- Tryptone ................................................................................................................... 5,00 g
- Peptic digest of meat ................................................................................................ 5,00 g
- Meat extract ............................................................................................................. 5,00 g
- Glucose ..................................................................................................................... 5,00 g
- Disodium phosphate ................................................................................................ 4,00 g
- Ferrous sulfate .......................................................................................................... 0,30 g
- Bismuth ammonium citrate........................................................................................ 1,85 g
- Sodium sulfite ........................................................................................................... 6,15 g
- Brilliant green ......................................................................................................... 25,0 mg
- Bacteriological agar................................................................................................. 14,70 g
pH of ready-to-use media at 25 °C : 7,6 ± 0,2.
PACKAGING
Dehydrated medium : BK004HA - 500 g bottle
DETECTION OF SALMONELLA TYPHI AND OTHER SALMONELLAE