Alkaline Saline Peptone Water is a medium for the enrichment of Vibrio spp. from food and water samples according to ISO 21872-1:2017 and ISO 21872-2:2007, and is not intended for use in the diagnosis of disease or other conditions in humans.
Originally described by Shread, Donovan & Lee as an enrichment broth for Aeromonas spp. and identified by Cruickshank as an effective medium for the enrichment of Vibrio spp., Alkaline Saline Peptone Water uses elevated pH and salt levels to provide a favorable environment for the enrichment of Vibrio spp.
Peptone provides essential vitamins, minerals, amino acids & nitrogen for growth requirements. Sodium chloride provides essential electrolytes for maintenance of the osmotic balance.
Sub-culture onto Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar (NCM0052) will be required.
| Formula | Liter |
| Peptone | 20.0 g/L |
| Sodium Chloride | 20.0 g/L |
Final pH: 8.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Preparation
- Dissolve 40 grams of the medium in one liter of purified water.
- Heat with frequent agitation to completely dissolve the medium, if necessary.
- Autoclave at 121°C for 15 minutes.
Product was previously known as LabM Product: LAB224 ASPW - Alkaline Saline Peptone Water (ISO)
Order Details
| Product # | Product Description |
| NCM0175A | Alkaline Saline Peptone Water (ASPW) (ISO), 500 g |
| NCM0175B | Alkaline Saline Peptone Water (ASPW) (ISO), 5 kg |
| NCM0175C | Alkaline Saline Peptone Water (ASPW) (ISO), 10 kg |
