DESCRIPTION:

Acid Hydrolysate of Casein is included in the composition of culture media intended for microbiological assays of vitamins and of tryptophan, as well as for the study of the resistance of bacteria to antibiotics, to sulfamides in particular. It is included in Mueller-Hinton Agar.

TYPICAL ANALYSIS

Physical characteristics :

- Appearance, color ....................................................................................................... off-white powder

- Solubility in water at 5 % ................................................................................................................. total

- pH of an aqueous solution at 5 % ........................................................................................... 5,5 ± 0,5

- Stability after autoclaving for 15 min at 121 °C ..............................................................................stable

Chemical characteristics :

- Biuret reaction ............................................................................................................................negative

- Total Nitrogen ...................................................................................................................... 7,5 ± 0,5 %

- α-amino Nitrogen.................................................................................................................. 5,5 ± 1,0 %

- Total carbohydrates ........................................................................................................less than 0,5 %

- Indole ........................................................................................................................................... absent

- Nitrites ......................................................................................................................................... absent

- Chlorides (expressed in NaCl) ....................................................................................... less than 45 %

- Calcium ....................................................................................................................................... 0,05 %

- Loss on drying .............................................................................................................. less than 6,0 %

Total amino acids (expressed in g per 100 g of product) :

- Aspartic acid ..................................................................................................................................... 4,4

- Threonine ......................................................................................................................................... 2,2

- Serine ............................................................................................................................................... 2,7

- Glutamic acid .................................................................................................................................. 12,5

- Proline .............................................................................................................................................. 6,1

- Glycine .............................................................................................................................................. 1,2

- Alanine .............................................................................................................................................. 2,0

- Valine ................................................................................................................................................ 3,9

- Cystine ................................................................................................................................ not assayed

- Methionine ........................................................................................................................................ 1,2

- Isoleucine ......................................................................................................................................... 2,4

- Leucine ............................................................................................................................................. 3,4

- Tyrosine ............................................................................................................................................ 0,6

- Phenylalanine ................................................................................................................................... 2,5

- Lysine ............................................................................................................................................... 5,6

- Histidine ............................................................................................................................................ 1,8

- Arginine ............................................................................................................................................ 2,2

- Tryptophan ....................................................................................................................................... 0,0

PACKAGING

Dehydrated medium : 
A1404HA - 500 g bottle

PEPTONE - PREPARATION OF CULTURE MEDIA