DESCRIPTION:

Tryptone USP is suitable for a wide variety of uses in general bacteriology for the preparation of culture media. As a result of its high tryptophan concentration, it is used for the detection of indole production. Lacking fermentable carbohydrates, it is included in media used to study sugar fermentations. Because of its compatibility with other ingredients, peptones or extracts, Tryptone USP is included in the composition of media intended for the growth and enumeration of bacteria in water, milk, other food products, pharmaceuticals and cosmetics. Combined with papaic digest of soybean meal, it is used for the preparation of Tryptone Soy Broth and Tryptone Soy Agar.

TYPICAL ANALYSIS

Physical characteristics :

- Appearance, powder color ................................................................................................. Cream-white

- Solubility in water at 5 % .................................................................................................................. total

- pH of an aqueous solution at 5 % ............................................................................................. 7,0 ± 0,7

- Stability at pH 7,0 after autoclaving for 15 minutes at 121 °C........................................................stable

Chemical characteristics :

- Biuret reaction ............................................................................................................................. positive

- Total Nitrogen..................................................................................................................... 12,5 ± 0,5 %

- α-amino Nitrogen................................................................................................................... 4,0 ± 0,1 %

- Indole.............................................................................................................................................absent

- Nitrites ...........................................................................................................................................absent

- Chloride (expressed as NaCl) .........................................................................................less than 1,0 %

- Calcium............................................................................................................................less than 0,1 %

- Sulfuric ash....................................................................................................................less than 17,0 %

- Loss on drying .................................................................................................................less than 6,0 %

Total amino acids (in g per 100 g of product) :

- Aspartic acid....................................................................................................................................... 6,9

- Threonine ........................................................................................................................................... 3,3

- Serine ................................................................................................................................................. 4,1

- Glutamic acid.................................................................................................................................... 18,5

- Proline ............................................................................................................................................... 9,0

- Glycine................................................................................................................................................ 3,2

- Alanine................................................................................................................................................ 3,1

- Valine.................................................................................................................................................. 6,1

- Cystine......................................................................................................................................not dosed

- Methionine.......................................................................................................................................... 2,4

- Isoleucine ........................................................................................................................................... 4,9

- Leucine ............................................................................................................................................... 8,1

-Tyrosine.............................................................................................................................................. 1,5

- Phenylalanine..................................................................................................................................... 4,9

- Lysine ................................................................................................................................................. 7,6

- Histidine............................................................................................................................................. 2,9

- Arginine .............................................................................................................................................. 3,2

- Tryptophan ......................................................................................................................................... 1,2

Microbiological characteristics :

- Indole production......................................................................................................................... positive

- H2S production ........................................................................................................................... positive

- Acetylmethylcarbinol production ................................................................................................. positive

- Detection of fermentable sugars ............................................................................................... negative

PACKAGING

Dehydrated medium : 
A1401HA - 500 g bottle
A1401GC - 5 kg drum